Interferometric multilayered nanomaterials for imaging unlabeled biorecognition events

Abstract There is an intense scientific activity on nanomaterials for biosensing, supported by their great potential to conceive powerful applications in chemistry, biology, and medicine. In addition meet the required analytical parameters, social impact of these advances also highly influenced cost, simplicity, portability aspects, which are critical label-free systems. This paper addresses design, development, experimental assessment multilayered transduce unlabeled biorecognition assays as both constructive destructive interferences, means simple effective imaging setups. The materials rely superposed nanometric films metals (gold Ag-In-Sb-Te alloy) a dielectric (zinc sulphide) deposited polymeric substrate. They tailored display maximal (constructive destructive) interferences immunoassays performed surface, then fabricated sputtering characterized focused ion beam scanning electron microscopy. Herein we address development optical setup that exploits standard DVD laser units scan reflected interferometric response microarrayed immunoassays. bioanalytical performance approach experimentally assessed using representative model immunoassay (BSA/anti-BSA) competitive quantify low molecular weight drug (sulfasalazine), reaching detection limits 460 11 ng mL−1 targets, respectively. study explores two alternative one-shot approaches provide insights into simpler faster strategies biosensing.